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高效液相色谱分析银杏叶提取物中总黄酮甙

ALANYSIS OF THE FLAVONOIDS FROM GINKGO BILOBA EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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【作者】 王爱民

【Author】 Wang Aimin (Analysis and Testing Center of Guizhou Normal University, Guiyang 550001)

【机构】 贵州师大分析测试中心

【摘要】 将银杏叶提取物经酸水解得到其中黄酮甙元槲皮素、山萘酚和异鼠李素,采用高效液相色谱、YWG-C18柱,甲醇:水(含04%磷酸)为流动相,UV360nm检测,以槲皮素为对照品计算出银杏叶提取物中总黄酮甙含量。槲皮素浓度在001~02mg/ml之间,浓度与峰面积呈直线关系,相关系数为09992,回收率为974%,相对标准偏差161%。该方法简便、准确,易于银杏叶提取物的质量控制。

【Abstract】 Hydrolysis of the extracts of the ginkgo biloba L obtains aglycones quercentin, kaempferol and isorhamnetin HPLC method has been uesed in separation and determination of aglycones on YWG-C 18 column with methanol-0 4% phosporic acid as eluents, which has been detected by ultraviolet detector at 360 nm A good linear relationship of quercentin has been found in the range of 0 01-0 20 mg/ml The average recovers are 97 4% The relative standard deviation is 1 61% This method is sample and accurate for quality control of the extracts of the ginkgo biloba L

  • 【文献出处】 贵州师范大学学报(自然科学版) ,JOURNAL OF GUIZHOU NORMAL UNIVERSITY(NATURAL SCIENCE) , 编辑部邮箱 ,1997年03期
  • 【分类号】O657.7
  • 【被引频次】3
  • 【下载频次】213
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