节点文献
植物总DNA和核DNA提取及其纯度的研究
STUDY ON THE EXTRACTION AND PURITY OF PLANT TOTAL DNA AND NUCLEAR DNA
【摘要】 采用氯份—异戊醇—RNA酶法及其改良法从小麦、燕麦黄化苗和大豆胚轴中提取植物总DNA和核DNA,结果表明:DNA纯度A260/A280≥1.80,A260/A230≥2.00,DNA片段为50Kb左右,均符合DNA分子育种的要求。
【Abstract】 Adopt the method of chloroform-isopentanol -Rnase and its method of improved from yellow seedling of wheat, oat and plumular axis of soybean extracted plant total DNA or nuclear DNA, the result showed: The purity of DNA A260/A280 1. 8, A260/A280 2.00,DNA franment about 50KB, accord with demand of DNA molecular breeding.
【关键词】 植物总DNA;
核DNA;
DNA纯度;
分子育种;
【Key words】 plant total DNA nudear DNA DNA purity moclcular breeding;
【Key words】 plant total DNA nudear DNA DNA purity moclcular breeding;
- 【文献出处】 宁夏农学院学报 ,JOURNAL OF NINGXIA AGRICULTURAL COLLEGE , 编辑部邮箱 ,1997年03期
- 【分类号】Q942.2
- 【被引频次】38
- 【下载频次】835