【摘要】 通过激光诱变筛选，获得一株高产纤维素酶的突变株ＨＤ９４７８，在以稻草、麦麸为主要原料的固体培养基上进行发酵培养，所产纤维素酶的固体曲，以羧甲基纤维钠为底物的纤维素ＣＸ酶活力为３５００Ｕ／ｇ，以滤纸为底物的纤维素ＣＬ酶活力为２６０Ｕ／ｇ，以水杨素为底物的β－葡萄糖苷酶活力为１２００Ｕ／ｇ，发酵培养的条件是：培养基起始ｐＨ６．０～７．０，培养温度２８～３２℃，培养时间７２ｈ。三种酶作用的最适ｐＨ分别为３．５，５．５和４．５，最适温度分别为６０，５５，６０℃，酶的ｐＨ稳定性与热稳定性为纤维素ＣＬ酶最高，纤维素ＣＸ酶次之，β－葡萄糖苷酶最差。更多还原

【Abstract】 A mutant strain HD9478 with high potency for cellulase production was obtained from industrial enzyme-producing strain which was screened by laser mutagenesis. In straw-wheat bran solid fermentation,its enzyme activity was 3 500 U/g on carboxyme thylcellulose-Na,260 U/g on filter paper and 1 200 U/g on salicoside. The optimum culture conditions were as follows:inifial pH6.0～7.0,temperature 28～32℃ and cutivation time 72 h. The optimal pH and temperature for cellulase reaction were pH3.5,5.5,4.5 and 60℃,55℃,60℃. The pH stability and thermal stability were studied. The results indicated that the stability of cellulase C L enzyme was the best and that of β-glucosidase was the worst.更多还原