【摘要】 目的：探讨纤维连接蛋白（ＦＮ）与大肠癌的关系。方法：应用灵敏度高、特异性强的生物素－卵白素酶标记复合物免疫组化技术，结合计算机图像分析系统，对正常大肠粘膜、大肠癌组织ＦＮ的分布进行了全面系统的定性、定量研究。结果：发现随着肿瘤分化程度的降低，细胞ＦＮ阳性标记的密度、阳性细胞百分率逐渐降低，平均灰度值逐渐升高；Ｄｕｋｅｓ分期由Ａ至Ｃ期，细胞ＦＮ阳性标记的密度亦逐渐降低，平均灰度值逐渐升高，而基膜ＦＮ和间质ＦＮ无明显变化。结论：表明细胞ＦＮ与大肠癌组织分化、侵袭和转移有关。更多还原

【Abstract】 Purpose To study the relationship between fibronectin and colorectal carcinoma. Methods By highly sensitive, specified avidin biotin peroxidase complex immunochemical technique combined with computer image analysis system, we investigated the distribution of fibronectin (FN) in normal colorectal mucosa and carcinoma qualitatively and quantitatively. Results The results showed that with the decrease of differentiation of carcinoma, the density and percentage of FN positive colorectal cells were gradually decreased and the average gray value was increased. For Dukes stage from A to C, the density of FN positive cells was also decreased and the average gray value was increased. No marked changes of FN were detected in the stroma and basement membrane. Conclusions The cellular FN is related to differentiation, invasion and metastasis of colorectal carcinoma.更多还原