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交联烯丙基葡聚精凝胶固定化脂肪酸的研究
STUDY ON THE ENTRAPMENT OF LIPASE IN THE COPOLYMER OF ALLYL DEXTRAN AND N, N’-METHYLENEBISACRYLAMIDE
【摘要】 本文研究了烯丙基葡聚糖和N、N'-亚甲基双丙烯酰胺经反相悬浮聚合包埋固定化脂肪酶的方法。考察了悬浮介质、交联度对固定化酶的影响,得出了包埋固定化脂肪酶的最佳条件;并测定了固定化脂肪酶的催化性能,确定了该固定化酶催化橄榄油水解的最适条件。
【Abstract】 Lipase was entrapped in the copolymer of allyl dextran and N, N’ methylenebisacrylamide via reverse suspension polymerization. The immobllization conditions, such as suspension medium, degree of cross-linking were optimized. The catalytic activity of the entrapped lipase in hydrolysis process of olive oil was investigated and optimal, hydrolysis conditions were determined.
【关键词】 脂肪酶;
固定化;
交联烯丙基葡聚糖凝胶;
【Key words】 Lipase; Immobilization; Crosslinked copolymer of allyl dextran;
【Key words】 Lipase; Immobilization; Crosslinked copolymer of allyl dextran;
【基金】 江苏省教委“青蓝工程”基金
- 【文献出处】 离子交换与吸附 ,ION EXCHANGE AND ADSORPTION , 编辑部邮箱 ,1997年05期
- 【分类号】TQ225.1
- 【被引频次】7
- 【下载频次】59