【摘要】 观察碱性纤维母细胞生长因子对大鼠主动脉内皮剥脱所致血管损伤修复的影响，以探讨碱性纤维母细胞生长因子在防治经文腔内血管成形术后再狭窄中的应用前景。在制作动物模型后，用放射免疫法测定了内皮到脱大鼠主动脉组织环一磷酸鸟苷和血浆内皮素的含量。结果发现，无论是早期或晚期给碱性纤维母细胞生长因子都能明显增强剥说血管对不同浓度的乙酸胆碱的舒张反应，促进一氧化氢的合成。表现为血浆一氧化氛含量和主动脉组织环一磷酸鸟苷合量升高，血浆内皮素浓度和血管壁内膜／中膜面积比值下降。由此可见，适当应用碱性纤维母细胞生长因子可促进内文功能的恢复，抑制内膜的过度增生而防止再狭窄。更多还原

【Abstract】 Aim To study the effects of basic fibroblast growth factor (bFGF) on the repairment of the injured intima by balloon denudation in rat aorta. Methods The male Wistar rats were divided into six groups as follows, two early treatment groups (a 7day-group and a 21 day-group ), one late treatment group, and three control groups including a simulated operation group. The intima of the aortae was denuded by using a balloon catheter. bFGF was injected into the tail veins of the animals on the 1st, 2nd and 3rd day (early treatment) and on 15th, 16th and 17th day after balloon denudation (late treatment ), respectively. The denuded aorta rings were perfused in the Krebs-Henseleit’s balance salt bath. The plasma NO levels were determined by the method described by Green and his colleagues, and the plasma endothelin levels and the cGMP content in the aortic tissue were measured by radioimmunoassay.ResultS Intravenous injection of bFGF, not only in the early treatment but also in the late treatment,could significantly promote the relaxation reaction induced by acetylcholine. The maximal relaxation mag nitude of the denuded aorta rings in the 7-day- treatment group and 21-day-treatment group were 2. 2 and 1. 7 times that of both control groups, respectively, and the maximal relaxation magnitude in the late treatment group was 2. 6 times as high as in control group.After denudation, the plasma NO2 levels of the 7-day and 21-day control group were lower by 12% and 27%, and the cGMP contents of both control groups were lower by 57% and 48%, whereas the plasma endothelin levels of both control groups were higher by 70% and 40%, respectively, than that of the simulated operation group. Meanwhile, the plasma NO2 levels of both early treatment groups were higher by 50% and 115%, and the cGMP content of both early treatment groups were higher by 106% and 100%, respectively, than that of the control groups, whereas the plasma endothelin levels were significantly lower.The results of the late treatment group were simular to that of the early treatment groups.Conclusion Using bFGF might promote the repairment of denuded endothelium, inhibit the overproliferation of denuded intima, and therefore might play a role in preventing restenosis of balloon denuded aorta.更多还原