【摘要】 为了研究调控小分子G蛋白（ras蛋白）以抑制血管平滑肌细胞对生长因子的反应机制，用合成的反斗ras寡聚脱氧核苷酸作用于碱性纤维母细胞生长因子以刺激培养的大鼠血管平滑肌细胞，通过鼠标胸腺嘧啶脱氧核苷酸掺入法及免疫细胞化学方法，来观察反斗ras聚脱氧核苷酸对在碱性纤维母细胞生长因子刺激下的大鼠血管平滑肌细胞DNA合成和增殖细胞核抗原的影响。结果发现，反义ras寡策脱氧核苷酸可明显抑制血管平滑肌细胞DNA的合成，作用12h和24h的抑制率分别为92％和90％；而相同浓度的正义链组仅呈现较弱的抑制作用（P＜0．01）．反义ras寡聚脱氧核苷酸亦可显著减少血管平滑肌细胞增殖细胞核抗原蛋白的表达．提示该策略的应用有可能解决以生长因子为始因引起的细胞增殖性疾病、经文腔内冠状动脉成形术后再狭窄、动脉粥样硬化及高血压等问题的临床防治．更多还原

【Abstract】 Aim in order to regulate the action of small molecular G protein (ras protein) for inhibition of smooth muscle cell (SMC) proliferation response to growth factor, basic fibroblast growth factor (bFGF).Methods Antisense oligomers, modified to prevent their own degradations, were used in a concentration of 6 pool/L introduced into the serum-free medium containing bFGF (100 mg/L) in which rat aortic SMC were cultured. By means of H-thymidine incorporation and immunocytochemistry methods, DNA synthesis and expression of proliferating cell nuclear antigen. (PCNA) in SMC were detected.Results These oligodeoxynucleotides (ODN), in a time-related manner, significantly lowered the DNA synthesis of SMC by more than 90% (compared with the control). When a same nucleotides sense ODN was used instead, showed only a little effect. lmmunocytochemistry indicated that the antisense ODN can markedly reduce expression of PCNA protein which is necessary for SMC proliferation. Conclusion Antisense G protein ODN strategies may be used to resolve clinical problems caused by growth factors initiated cell proliferation.更多还原