【摘要】 为了研究胰岛素样生长因子-1受体在血管平滑肌细胞生长调节中所起的作用，用其寡核苷酸反义链和配链对鼠主动脉平滑肌细胞的作用进行研究。用寡核苷酸反义链处理的血管平滑肌细胞中，氚标脱氧胸腺嘧啶核苷的掺入率较对照组下降64％，用结合实验测定胰岛素样生长因子-1受体数目下降52％。在用反义链处理细胞时，随加入胰岛素样生长因子-1浓度的增加，氚标脱氧胸腺嘧啶核苷掺入率也与剂量成反比的下降。反义键的剂量为20μg／L时，氚标脱氧胸腺嘧啶核苷掺入率下降64％。反义链处理的细胞对加入血管紧张素Ⅱ无反应。受体数目无变化。这一发现提示了配体一受体系统对血管平滑肌细胞反应的重要性。受体的密度对血管平滑肌细胞的生长起到关键作用。用反义链能使胰岛素生长因子-1受体数目减少是由于胰岛素样生长因子-1受体的mRNA减少，这一假说在核酸酶保护实验中得到证明。更多还原

【Abstract】 Aim In order to study the role of insulin-likegrowth of factor-1 receptor (IGFR) in the growth ofvascular smooth muscle cells(VSMC).Methods Antisense and mismatch of oligodeoxynu-cleotides (ODN) of IGFR were used to study smoothmuscle cell of mouse arteries.Results The incorporation of 3H-thymidine inVSMC handling by antisense of ODN decreased 64%compared with normal control group. The determi-nation of the receptor number in binding experimentdecreases 52% cell handing by as incubated differentconcentration of IGF-l (1 ~ 50 ng) caused a dose de-pendent increase in 3H-thymidine incorporation stimu-lated by 20 μg/L IGF-1 was reduced by 62% in anti-sense treated cells the effect of cells incubation of an-giotensin Ⅱ in antisense handling cells was not al-tered,the receptor quantities didn’t reduce.Conclusion This experiment showed the impor-tance of ligand-receptor system to VSMC. The den-sity of receptor p1ays a key role to the growth ofVSMC the decreasing of IGF-1 receptor numbercaused by antisense was due to the decreasing of mR-NA in IGF-1 receptor. This hypothesis has beenproved in the experiment of nudease protection.更多还原