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几种不同鹅膏菌毒素对真核生物RNA聚合酶Ⅱ抑制作用的比较研究
A STUDY OF THE INFLUENCE OF DIFFERENT AMENITA TOXINS ON RNA POLYMERASE Ⅱ IN EUKARYOTIC CELLS
【摘要】 用鹅膏菌属(Amanita)含α-毒伞肽的毒素粗提液培养新鲜绿豆,结果在36小时后,各种不同毒苗的毒素粗提液培养的绿豆生长情况有明显不同,用紫外吸收法测定蛋白质含量,发现毒素粗提液培养的绿豆细胞中蛋白质含量比蒸馏水培养的有明显下降,这表明α-毒伞肽的作用机理的确是通过抑制RNA聚合酶Ⅱ而寻致蛋白质合成减少。
【Abstract】 The Culture of Green beaus in extract solution of seven poisonous mushrooms resulted in different effects. There are obvious difference among the content of the proteins in Green beaus which were cultured in extract solution of seven poisonous mushrooms, and it is thought that α-amanitin in extract solution of these mushrooms can inhibit the function of RNA polymerase Ⅱ in eukaryoctic cells and further inhibit the synthsis of protein.
【关键词】 α-毒伞肽;
鹅膏菌;
RNA聚合酶Ⅱ;
抑制作用;
【Key words】 α-amanitin; Amanita mushroom; RNA polymerase Ⅱ; Inhibition;
【Key words】 α-amanitin; Amanita mushroom; RNA polymerase Ⅱ; Inhibition;
【基金】 国家科委重点课题!93(7)-03
- 【文献出处】 菌物系统 ,MYCOSYSTEMA , 编辑部邮箱 ,1997年04期
- 【分类号】Q93
- 【被引频次】11
- 【下载频次】184