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慢性乙型肝炎表面抗原携带者前S1基因的高度异质性
The quasispecies of preS 1 gene in a chronic HBsAg carrier
【摘要】 应用半巢式聚合酶链式反应（ＰＣＲ）从一例慢性ＨＢｓＡｇ携带者血清中扩增出ＨＢＶ前Ｓ１基因，将其克隆于噬菌体Ｍ１３ｍｐ１９中进行序列分析。结果发现：与同源性最好的ＨＢＶａｄｒ野生株相比，所测的１０个克隆均有替代和插入突变，９个克隆有缺失突变，１０个克隆的核苷酸变异率为５．０％～１７．０％，氨基酸的变异率为１３．０％～６０．０％；１０个克隆之间的核苷酸变异率为２．３％～２４．３％，氨基酸的变异率为１４．０％～６６．０％；１０个克隆中没有核苷酸序列一致的克隆。上述资料证实，慢性ＨＢｓＡｇ携带者体内的ＨＢＶ前Ｓ１基因具有高度的异质性。
【Abstract】 Half nested polymerase chain reaction(PCR) was used to amplify HBV preS 1 gene from sera of a chronic HBsAg carrier.PCR Products were cloned into M13mp19 vector for DNA sequencing. The results were as follows: 1. compared with the wild type HBV adr, all clones exhibited base insertion and substitution. Nine of 10 clones had base deletion. The mutation rate of nucleotides was 5.0-17.0%, and the difference rate of amino acids was 13.0-60.0%; 2. compared between the clones themselves,the base mutation rate was 2.3-24.3%, and the difference rate of amino acids was 14.0-66.0%. There was no clone showing entirely identical sequences in all 10 clones. These data confirmed that quasispecies of HBV preS 1 gene existed in this chronic HBsAg carrier.
【Key words】 HBV； gene mutation； preS 1 gene； polymerase chain reaction；
- 【文献出处】 军事医学科学院院刊 ,BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES , 编辑部邮箱 ,1997年04期
- 【分类号】R512.62
- 【被引频次】2
- 【下载频次】20