【摘要】 对黑曲霉与酿酒酵母原生质体的形成和再生条件进行了研究，得出黑曲霉原生质体形成和再生的较优条件为：混合酶浓度0．8％，酶解时间2小时，酶解温度34℃。酿酒酵母原生质体形成和再生的软化条件为：蜗牛酶浓度2％，酶解时间90分钟，酶解温度30℃。更多还原

【Abstract】 Through the cross cut tests the suitable condition of protoplast formation and regeneration of strains A. niger and S. cerevisiae were studied. The suitable condition of protoplast formation and regeneration of A. niger and S. cerevisiae are combined enzyme 0.8%,zymolytic time 2 h, temperature 34℃ and snail enzyme 2%, zymolytic time 1 .5 hr, temperture 30℃, respectively.更多还原