【摘要】 用聚丙烯酰胺垂直平板电泳技术研究了不同底物（α－萘醋酸酯和β－萘醋酸脂）、不同偶联剂（固蓝ＲＲ盐和固蓝Ｂ盐）、ｐＨ及温度条件对大麦酯酶同工酶电泳显色的影响。结果为：（１）绝大多数酶带可用α－萘醋酸酯或β－萘醋酸酯显色，极少数酶带只能用α－萘醋酸酯显示，混合底物显色时，酶带数目与α－萘醋酸酯底物显色相同，只是酶带颜色有差异；（２）用固蓝ＲＲ盐作偶联剂染色与用固蓝Ｂ盐相比，多数酶带的相对染色强度提高；（３）ｐＨ６．４比较适合酯酶同工酶染色，且以３７℃１０ｍｉｎ为最佳。表明各电泳酶带染色强度的相对差异并不一定反映各同工酶本身含量、酶活性的相对高低。更多还原

【Abstract】 The influence was studied on the staining of barly esterase isoenzyme bands separated by the method of thin layer polyacrylamide gel of different substrates (αnaphthylacetate and βnaphthylacetate) and different coupling agents (fast blue RR salt and fast blue B salt) and pH. The results obtained were as follows: (1) Most bands were stained by using αnaphthylacetate or βnaphthylacetate as a substrate; some bands were stained only by with αnaphthylacetate as a substrate; using mixed substrate of αand βnaphthylacetate, there were the same number of electrophoretical bands as with αnaphthylacetate as a substrate, but the colour of bands were different. (2) Stained by using fast blue RR salt as a coupling agent, the relative staining strength of some bands was increased, compared with using fast blue B salt as a coupling agent. (3) The optimal pH value for staining was 6.4. These suggested that the relative staining strength of the electrophore tical bands didn’t necessarily represent the relative activities or amount of esterase isoenzymes.更多还原