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兔致病性肺炎霉形体分离培养和鉴定
Isolation and Identification of Pathogenic Mycoplasma from Rabbit Pneumonia
【摘要】 采集临床表现流鼻液、打喷嚏等慢性呼吸道疾病症状长毛兔的鼻液,临床剖检有可疑病肺灶,分别接种牛肝汤培养液。置37℃恒温箱培养24小时至48小时,用培养物涂片,瑞氏染色,显微镜检查。经牛肝汤培养,共分离出兔肺炎霉形体38株。按国际ICSB霉形体分类委员会建议的有关内容初步进行了鉴定。证明其是兔致病性的肺炎霉形体
【Abstract】 Respiratory secretions and lung samples with red infarcts were collected from rabbits with coryza.The samples were cultivated in beef liver infusion at 37℃ for 24 to 48 hours.After cultivation,38 mycoplasma strains were obtained.These isolates were identified according to the methods suggested by ICSB and proved to be pathogenic mycoplasma of rabbit pneumonia.
【关键词】 兔肺炎;
霉形体;
分离培养;
鉴定;
【Key words】 rabbit pneumonia; pathogenic mycoplasma; isolation; identification;
【Key words】 rabbit pneumonia; pathogenic mycoplasma; isolation; identification;
【基金】 江苏省科委资助
- 【文献出处】 江苏农业学报 ,JIANGSU JOURNAL OF AGRICULTURAL SCIENCES , 编辑部邮箱 ,1997年02期
- 【分类号】S858.291.05
- 【下载频次】33