The mixed solution of 5'-GMP and 5'-CMP could be obtained when Lentinus edodes (Berk)Sing fermented mash is handed by 732 positive Resin at a pH value 2. 5 ~3.0. As pH value was up to 4. 0 ~4. 5 and using 717 negative Resin, 5'-GMP was absorped. Then using 3% NaCl solution as a solvent, the 5'-GMP was separated. Fermented mash was ground by colloid mill to berak the cell wall. After that there were 3 treatments: autolysis, enzyme (phosophodiesterase) digestion and autolysis plus enzyme digestion. The 5'-GMP...