【摘要】 目的:研究血管成形术后平滑肌细胞中Fos、热休克蛋白—70的表达及意义.方法:将10只新西兰家兔随机分为正常对照组(血管数为n=4),血管成形术组(n=8),血管成形术+低分子量肝素组(n=8),以2F PTCA导管对两侧髂动脉进行血管成形术,术后1小时、2小时取血管成形后血管进行Fos、热休克蛋白—70免疫组化染色.结果:正常动脉内未见Fos表达,血管成形术后1小时部分平滑肌细胞阳性,2小时表达增多;正常动脉内热休克蛋白—70位于平滑肌细胞胞浆内,血管成形术后1、2小时平滑肌细胞核呈阳性;血管成形术+低分子量肝素组与单纯血管成形术组结果相同.结论:Fos于血管成形术后早期出现,与SMC增生迁移有关,低分子量肝素不影响此过程.首次发现血管成形术后热休克蛋白—70迅速增加,可能是一种对抗血管成形术损伤的保护机制.更多还原

【Abstract】 In this paper,we studied the expression and possible role of Fos and Hsp -70 in the rabbit iliac smooth muscle cells (SMCs) after angioplasty. Rabbits were divided at random into the control group (n = 4),the PTA group (n = 8) and the PTA+LMWH group (n = 8). At 1 and 2 hours after PTA,the vessels were examined by immuno -histochemistry of Fos and Hsp -70. The results were as follows;Fos expressed in SMCs at 1 hour and increased at 2 hours after angioplasty;Hsp - 70 increased in the nuclei of SMCs at 1 and 2 hours. The early expression of Fos may be related to the proliferation and migration of SMCs. The increasing of Hsp -70 may be a protective mechanism of SMCs for the injury after angioplasty.更多还原