【摘要】 为了研究上游信使系统中两个重要激酶——蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）和蛋白激酶Ａ（ｐｒｏｔｅｉｎｋｉｎａｓｅＡ，ＰＫＡ）与周期进程的相关性。采用胸腺嘧啶核苷（ｔｈｙｍｉｄｉｎｅ，ＴｄＲ）双阻断和笑气（Ｎ２Ｏ）Ｍ期阻断相结合的方法，获得同步化的Ｇ２和Ｍ期细胞，并以此为模型，检测了信使系统中两个重要的蛋白激酶ＰＫＡ、ＰＫＣ以及Ｍ期引擎分子ＣＤＣ２在ＨｅＬａ细胞Ｇ２／Ｍ和Ｍ／Ｇ１过程中激酶活性变化情况，结果显示，ＰＫＣ和ＰＫＡ与ＣＤＣ２活性变化分别呈正负相关性。更多还原

【Abstract】 おsing the method of thymidine (TdR) double arresting and N 2O arresting, synchronized G2 and M phase cells were obtained. Activity of PKA, PKC and CDC2 were assayed using this synchronized cell model. It indicated that PKA negatively while PKC positively correlate with CDC2 in G2/M/G1 progressing of HeLa cells.更多还原