【摘要】 采用ＳｅｐｈａｄｅｘＧ５０、ＣＭ－Ｃｅｌｕｌｏｓｅ３２柱层析，从中华眼镜蛇中分离出神经生长因子（ＮｅｒｖｅＧｒｏｗｔｈＦａｃｔｅｒ，ＮＧＦ）。经ＳＤＳ－聚丙烯酰胺凝胶电泳及Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ法证明所得到的ＮＧＦ为单一组分，相对分子质量约为１．３×１０４．经凝胶等电聚焦电泳测得ＮＧＦ等电点ＰＩ约为７．０左右．经ＨＰＬＣ及电泳图像分析系统测得纯度为９８％．此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明，具有促进神经纤维生长的活性．将所纯化的ＮＧＦ免疫大白兔获得抗血清，经ｐｒｏｔｅｉｎＡ－Ｓｅｐｈａｒ－ｓｏｅＣＬ－４Ｂ亲和层析柱纯化获得了较为单一的抗ＮＧＦＩｇＧ更多还原

【Abstract】 Nerve Growth Factor (NGF) from the venom of Naja naja atra cantor was purified by Sephadex G50,CM Cellulose 32 and HPLC column chronatography.It was proved to be homogeneous by polyacrylamid lab gel electrophoresis,isoelectrofocusing electrophoresis, SDS polyacrylamid lab gel electrophoreis,and western biotting.Isoelectrofocusing point is about 7.0.It stimulates in vitro nerve fiber growth of dorsal root ganglia of 8 day chicken embryo.High titer antisera was obtained by injecting NGF in male rabbits.Homogeneous IgG was obtained from the antisera by protein A sephorose CL 4B affinity column chromatography.更多还原