【摘要】 将pSC101质粒上的Par区域（控制质粒分配的基因座）克隆到含有天冬氨酸酶基因的质粒pXZ70上，构建成重组质粒pZZ1，将pZZ1质粒转化入大肠杆菌JM109细胞中，得到一株质粒可稳定遗传的重组子细胞，培养100代后，含质粒菌数仍在90％以上．更多还原

【Abstract】 The Par region of pSC101 plasmid was inserted into pXZ70 plasmid carrying aspartase gene. The new plasmid pZZ1 was constructed. The pZZ1 plasmid was trasformed into E. colt JM109 cells. The recombinat cells, which were stable and genetic, were obtained. After 100 generations, the number of cells containing plasmid pZZ1 was more than 90%.更多还原