Iron components in PS I particles of spinach by Mossbauer spectroscopy

【摘要】 <正> Mossbauer spectrum measured for the iron components of photosystem Ⅱ (PS Ⅱ ) particles of spinach is a superposition of 4 doublets. Quadrupole splitting and chemical shifting of doublets I -IV are characteris-tics of proteins with oxidized cytochrome b-559, reduced cytochrome b-559, Fe3 + -Q complex and Fe2+ -Q complex re-spectively . After the PS II particles are treated with La3+ , two doublets of Fe2+ disappear and Fe2+ is converted into Fe3+ , indicating that the reduced cytochrome b-559 has been converted into the oxidized cytochrome b-559, and Fe2+-Q complex into Fe3+-Q complex. The Mossbauer spectrum of PS II particles treated with La3+ and Ca2+ shows that Ca2+ can weaken the inhibitory effect of La3+ in part, and a portion of the reduced cytochrome b-559 and Fe-Q complex still exist.更多还原

【Abstract】 Mossbauer spectrum measured for the iron components of photosystem Ⅱ (PS Ⅱ ) particles of spinach is a superposition of 4 doublets. Quadrupole splitting and chemical shifting of doublets I -IV are characteris-tics of proteins with oxidized cytochrome b-559, reduced cytochrome b-559, Fe3 + -Q complex and Fe2+ -Q complex re-spectively . After the PS II particles are treated with La3+ , two doublets of Fe2+ disappear and Fe2+ is converted into Fe3+ , indicating that the reduced cytochrome b-559 has been converted into the oxidized cytochrome b-559, and Fe2+-Q complex into Fe3+-Q complex. The Mossbauer spectrum of PS II particles treated with La3+ and Ca2+ shows that Ca2+ can weaken the inhibitory effect of La3+ in part, and a portion of the reduced cytochrome b-559 and Fe-Q complex still exist.更多还原