【摘要】 采用荧光测量方法研究成功的海水叶绿素a现场测量仪，是用于海上现场实时测量海洋中浮游植物体内叶绿素a含量的新型海洋仪器。本仪器采用低功耗、高强度、低重复频率、窄脉冲宽度的脉冲氙灯，为激发光源及相应的脉冲检测技术测量荧光信号。该仪器可在海表层至50m水深内任一日光强度下工作；测量海洋中浮游植物体内叶绿素a的浓度范围为1×10-6—1×10-9g／cm3；当仪器走航拖曳速度为2m／s时，其空间分辨度为1m，仪器具有海上现场测量实用价值。更多还原

【Abstract】 Ths article describes an in-situ instrument of the chlorophyll-a inseawater which has been successfully developed on the basis of fluorometry. It ismainly used for measuring the chlorophyll-a concentration in phytoplankton in theseasite so that the distribution of sea organisms resources can be assessed. Theinstrument use the pulsed xenon lamp as the excited light resources which has thecharacteristies of low power consumption, high intensity, low repetition rate andnarrow puse width and also use the relevant pulsed detecting technology (i. e. theBoxcar integrating circult) for detecting the fluorescent signal. The signal strength isratioed against the lamp intensity for each flash, by using the detecting methods ofdouble light route and double channel at the same time, so that the accuracy of everymeasuring figure can be ensured, and signal variation due to undulation of the pulsedxenon lamp output intensity can be effectively eliminated. In order to effectivelyseparate the fluorescence produced by the chlorophyll-aof the phytoplankton in thesea which is shone by the excited light and the scatter light from the excied light weuse high block coefficient narmw band filters, the center wavelength of the excitationfilter is 440nm, and the fluorescent emitapce filtors center wavelength is 685nm. Inthis way, the fluorometric signal strength which is received by the receivers can beensured to reflect the fluorescence of chlorophyll-ain the phytoplankton, andfurthermore the interference from the inherent fluorescence in the seawater can beeffectively prevented.The instrument can work from the sea surfuce to a maximum depth of 50m, atany sunlight intensity, for detecting concentration range of chlorophyll-αin thephytoplankon in the ocean from 1 ×10-6- 1×10 9 g / cm3, when the instrument istowed at a speed of 2m/ s, its space resolution is 1m, and this is of practical value to us.更多还原