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电喷雾电离质谱测定细胞色素b₅及其定点突变蛋白

Molecular Weight Determination of Cytochrome Tb₅ and Lb₅ and Their Mutant Proteins by Electrosprary Ionization Mass Spectrometry Technique

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【Author】 XU Yong - Zhen ZHANG Yi - Bing(State key Laboratory of Bio - organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, The Chinese Academy of Sciences, Shanghai, 200032)SUN Yu-Long WANG Yun - Hua HUANG Zhong - Xian(Department of Chemistry, Fudan University, Shanghai, 200433)

【机构】中国科学院上海有机化学研究所生命有机化学国家重点实验室；复旦大学化学系；复旦大学化学系上海200032；上海200032；上海200433；上海200433；

【摘要】本文利用电喷雾电离质谱技术对胰蛋白酶酶解的细胞色素b₅蛋白Tb₅及脂肪酶酶解的细胞色素b₅蛋白Lb₅及它们的定点突变蛋白共八个样品的分子量进行精确测定,证实定点突变的试验是成功的.更多还原

【Abstract】 The determination of molecular weights of cytochrome Tb5 and Lb5 and six mutant proteins has been succeeded by using Electrosprary Ionization Mass Spectrometry (ESIMS) technique.The successful site- directed mutagenesis of cytochrom b5 has been proved.更多还原

【关键词】细胞色素b₅；定点突变；电喷雾电离质谱；分子量测定；

【基金】国家自然科学基金资助课题