【摘要】 报道了新荧光衍生试剂Ｎ－羟基琥珀酰亚胺－α－萘乙酸酯（ＳＩＮＡ）的合成。ＳＩＮＡ的乙腈溶液在λｅｘ／λｅｍ＝２８０ｎｍ／３３９ｎｍ处有最强荧光。在碱性水溶液中，ＳＩＮＡ易与胺、氨基酸等氨基化合物进行衍生反应，生成有荧光的酰胺化合物，过量试剂在１ｈ内完全水解为相应的α－萘乙酸，衍生产物及水解物易在反相键合色谱柱上分离。研究表明，ＳＩＮＡ易制备，性能稳定，与氨基化合物易衍生，可用于柱前衍生ＨＰＬＣ分离－荧光检测氨基酸、生物胺等生物活性物质。更多还原

【Abstract】 A new fluorescence derivatization reagent for amino compounds, N hydroxysuccinimidyl α naphthylacetate(SINA),is easily prepared by the reaction of α naphthylacetic acid with N hydroxysuccinimide in the presence of dicyclohexylcarbodiimide SINA in acetonitrile presents strong fluorescence at λ ex /λ em =280nm/339nm The reagent can readily react with amino compounds to give the corresponding fluorescent amides, which can be separated on reverse phase column SINA is found to be a promising derivatization reagent for analysis of amino compounds by HPLC with fluorescence detection更多还原