【摘要】 根据NDV、IBV二种病毒混合毒种的最适稀释比例,将混合毒种接种9¹0日龄鸡胚尿囊腔内（简称同胚培养）,使接种鸡胚96h二种病毒毒价同时达到高峰,0.1ml尿囊液含:NDV≥10⁸EID₅₀、IBV≥10₇EID₅₀.收获同胚培养的胚毒,制备油乳剂灭活疫苗.用三批疫苗分别免疫18日龄雏鸡,免疫后4周二联疫苗的有效抗体分别为:ND血凝抑制抗体（HI）≥1:16、IB中和抗体（VNT）≥1:8,抗体持续时间可达16周;攻毒保护率分别为:ND 8/8、IB 6/8以上,获得十分理想的效果.更多还原

【Abstract】 NDV and IBV were mixed according to the optimal dilution proportion, then the viruses were inoculated into chicken embryos at 9 or 10-dayage. the titres of two viruses both reached the peak at 96h, the allantoic fluid contained NDV≥108EID50 and IBV ≥107EID50 per 0. 1 ml. The viruses were harvested, and the inactivated oil-adjuvant vaccines were prepared. The chickens were vaccinated at 18-day-age,ail chickens had HI titres of ND ≥1:16 and neutralizing antibody of IB≥l:8 in 4 weeks after vaccination. The duration of immunity lasted 16 weekd, and the protection rate was 8/8(ND) and 6/8(IB) respectively.更多还原