【摘要】 采用单克隆抗体ＤＯ－１和免疫组化Ｓ－Ｐ法检测了口腔鳞癌和癌前病变组织中Ｐ５３蛋白表达。结果显示，正常口腔粘膜和轻、中度上皮异常增生中Ｐ５３蛋白阴性；３３．３％的上皮重度异常增生和５６．８％的鳞癌Ｐ５３表达阳性，癌组织中Ｐ５３表达与其分化程度、淋巴结转移和临床分期有关。由此作者认为，ｐ５３基因突变是口腔粘膜癌变过程中的早期事件并与口腔癌的进展有关，检测Ｐ５３蛋白有助估计口腔粘膜癌变可能性及口腔癌预后的潜在价值。更多还原

【Abstract】 After obtaining the results of p53 gene mutation by a silver staining method to polymerase chain reaction single strand conformation polymorphism (PCR SSCP) analysis, P53 protein staining and proliferation cell nuclear antigen (PCNA) index by double blind method, multivariate correlation analysis two by two showed that PCR_SSCP, P53 positive protein staining and PCNA index had markedly correlation not only in oral precancerous lesions (OPL) but also in primary sites and reginoal metastastic lymph nodes of oral squamous cell carcinomas (OSCC). This indicated that the nature of the three indexes was the same. After introducing the sex and age of patients which have been proved to be related to the initiation and development of OPL and OSCC according to other researchers and are easy to be obtained in clinics, multivariate discriminatory analysis was carried on and three groups of discriminatory equations were gotten about the pathological grading of OPL and OSCC and metastasis condition of OSCC. the accuracy of these equations was 82.5%, 78% and 80%, individually. This suggests that the equations have applicable potentiality already.更多还原