【摘要】 选用 SD1～ 3d乳鼠颅顶骨分离培养鼠体外成骨细胞 ,并对分离的鼠颅顶骨成骨细胞做多方面的生物学特性鉴定。结果表明 :鼠体外成骨细胞具有体内成骨细胞的形态特征、碱性磷酸酶活性以及合成、分泌骨形成蛋白等功能 ,并能在体外发生钙化 ,从而证实建立的鼠成骨细胞具有体内成骨细胞的功能和特性 ,可用于硬组织替代材料的细胞相容性研究 ,亦可用于与骨组织有关的医学基础实验更多还原

【Abstract】 Osteoblasts were isolated from Sprague Dawley rats of 1~3 days on the frontal parietal bones and were confirmed by testing for multiple biological features. The results showed that rat osteoblasts in vitro had the same morphological feature, ALP activity, the function of synthetic BMP as in vivo and it had the ability of calcification in vitro. The results proved that these rat osteoblasts in vitro had the same function and features as in vivo and it could be used to study cytocompatibility on replacement materials of human body’s hard tissues and biomedical to make experiments related to bone tissues.\;更多还原