【摘要】 利用共价偶联剂 SPDP将纯化的变形链球菌表面蛋白抗原 P1与霍乱毒素 B亚单位 (CTB)或前霍乱原类毒素 (PCG)分别进行偶联并进行偶联效果鉴定 ,以获取更为有效的防龋抗原。结果 :所得偶联物 P1- PCG,P1- CTB同时具有与神经节苷脂 GM1特异结合的特点 ,又具有 P1的免疫特性 ,为进一步进行免疫防龋实验提供了有效的抗原。更多还原

【Abstract】 To make an effeetive antigen to prevent dental caries, the surface protein antigen P1(Ag Ⅰ/Ⅱ) was purified and identified by the rabbit anti Ag Ⅰ/Ⅱ serum and was covalently conjugated to CTB and PCG by SPDP respectively. GM1 ELISA showed that both the conjugated P1 CTB and P1 PCG possess the ability to bind GM1 specifically without losing the antigenicity of P1. This results showed that the conjugated antigens could be used in the immune prevention against dental caries.\;更多还原