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外源DNA处理的草鱼对草鱼出血病毒的抗性观察

Sensibility of transgenic grass card on hemorrhage virus (GCHV) of grass card

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【作者】 章怀云萧克宇陈韬萧调义杨四秀何灵

【Author】 Zhang Huaiyun Xiao Keyu Chen Tao etc.

【机构】 湖南农业大学动物科学技术学院

【摘要】 用草鱼出血病毒人工感染经鲤鱼总DNA处理的草鱼和普通草鱼,在剂量为0.2mL/尾时,前者的LD50较为一致,为10-2.97,后者的为10-3.56~10-3.72,前者比后者的抗病力强3.89~5.89倍,潜伏期长1~2d,死鱼持续时间短1~2d.证明经鲤鱼总DNA处理的当年草鱼对草鱼出血病病毒的抗性显著增强

【Abstract】 Transgenic grass carp and common grass carp were artificially infected with grass hemorrhage virus.The LD 50  of the former is 10 -2.97 ,the latter is 10 -3.56 ~10 -3.72 when the same doses of 0.2 mL per capita were given.The antiviral ability of the former is 3.89 ̄5.89 times higher.The lantent period is 1 ̄2 d longer and the death duration is 1 ̄2 d shorter than that of the latter.The result suggests that the antiviral ability to carp hemorrhage virus of transgenic grass carp with carp general DNA is significantly increased.

【基金】 湖南省科委资助
  • 【文献出处】 湖南农业大学学报 ,JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1997年02期
  • 【分类号】S941.411
  • 【被引频次】19
  • 【下载频次】75
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