【摘要】 采用ＮａＩＯ４氧化鸟亲和素（Ａｖ）的寡糖链产生醛基后，与胺偶联，紫外证明每个Ａｖ偶联了５—６个胺基团。用丁二酸酐（ｓｕｃ）同Ａｖ上的赖氨酸残基酰基化反应，得到ｓｕｃ－Ａｖ等电点（２．５７）显著低于Ａｖ的等电点（１０．５３）。１２５Ｉ标记的各种Ａｖ衍生物和生物素的结合能力明显不同。更多还原

【Abstract】 The method of oxidation of the oligosaccharide on the avidin by NaIO 4 leads to the generation of aldehyde groups which are further reacted with octopamine. The conjugation ratio of 5.76 octopamine per Av is testified by UV spectrum. The isoelectric point (PI) of Av is remarkably reduced by conjugating succinic acid moieties at lysine residues of Av (PI10). The PI of suc-Av is 2.57. The combining capabilities with biotin of labelled Av derivants are greatly changed更多还原