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过氧化氢酶活性及活性抑制的紫外分光光度测定

THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY

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【作者】 徐镜波袁晓凡郎佩珍

【Author】 Xu Jingbo Yuan Xiaofan Lang Peizhen(Department of Environmental Science, Northeast Normal University, Changchun, 130024)

【机构】 东北师范大学环境科学系东北师范大学环境科学系 长春130024长春130024

【摘要】 在体内实验条件下,测定了鲤鱼肝脏过氧化氢酶的活性.实验结果表明,过氧化氢酶的紫外分光光度法是可靠的.活性抑制结果表明,对-二硝基苯(p-DNB)对鲤鱼肝脏过氧化氢酶均有明显的抑制作用,EC50值为0.0807mg·1-1.鲤鱼肝脏过氧化氢酶可作为一项指标用于生态毒理学研究.

【Abstract】 Liver catalase (Ct) activities of carp were measured by ultraviolet spectropho-tometry and the results were reliable. The effect of p-DNB on Ct of carp liver were studied in Vivo. The results showed that p-DNB exhibited fairly strong inhibition to Ct of carp livers. A satisfactory straight line was obtained by plotting the probits of inhibition percentage of Ct against the logarithmic concentration of toxicants, The concentration causing 50% inhibtion of p-DNB to Ct carp liver was 0. 087mg·I-1. It was proposed that the effect of toxicant on Ct could be used as an index of ecotoxicolgical assessment.

【关键词】 鲤鱼肝脏过氧化氢酶酶活性抑制
【Key words】 carp livercatalase (Ct)enzymic activityinhibition.
【基金】 国家自然科学基金资助课题
  • 【文献出处】 环境化学 ,Environmental Chemistry , 编辑部邮箱 ,1997年01期
  • 【分类号】O657.3
  • 【被引频次】257
  • 【下载频次】2854
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