【摘要】 本研究采用抗ＣＤ３单克隆抗体（ＣＤ３ＭｃＡｂ）辅以少量的基因重组人白细胞介素２（γＩＬ—２）和植物血凝素（ＰＨＡ）诱导外周血淋巴细胞，成功地研制出具有抗瘤活性的ＣＤ３ＭｃＡｂ激活的杀伤细胞（ＣＤ３ＡＫ细胞）。结果表明：微量的ＣＤ３ＭｃＡｂ辅以少量的γＩＬ—２和ＰＨＡ就能诱导并大量扩增ＣＤ３ＡＫ细胞。３０ｎｇ／ｍｌ的ＣＤ３ＭｃＡｂ就能一次性激活ＣＤ３ＡＫ细胞，当ＣＤ３ＭｃＡｂ浓度提高到３００ｎｇ／ｍｌ时，ＣＤ３ＡＫ细胞的扩增能力明显高于ＬＡＫ细胞；ＣＤ３ＡＫ细胞是以ＣＤ３＋、ＣＤ８＋和ＣＤ４＋细胞为主的异质性细胞群，ＣＤ３＋和ＣＤ８＋细胞百分率随培养时间延长而增加，培养第４天至第１６天的ＣＤ３ＡＫ细胞均有很强的杀瘤活性，而最佳时期在第７天至第１０天；其体外杀瘤活性明显高于ＬＡＫ细胞（Ｐ＜０．０５）。本实验研究表明：ＣＤ３ＡＫ细胞是继ＬＡＫ、ＴＩＬ后又一更为有效的杀瘤细胞。更多还原

【Abstract】 With trace CD 3McAb plus rIL-2 and PHA,perpheral blood lymphocyte was induced and the anti-CD 3 monoclonal antibody activated killer cells(CD 3AK)were prepared.The results showed that CD 3AK were stimulated by trace CD 3McAb plus rIL-2 and PHA,and were proliferated on a large scale.CD 3McAb can activated CD 3AK at one stroke in the concentration of 30ng/ml.When CD 3McAb concentration was 300ng/ml high,the proliferation of CD 3AK cells was far more than that of LAK cells. CD 3AK cells largely blong to CD 3 +,CD + 4and CD 8 + cells.The percentage of CD 3 +and CD 8 + cells increased with the culture time elongation,CD 3AK have high killing activity after culture for 4-16 days.The period of highest killing activity was in culture for 7-10 days,and its anti-tumor activity in vitro was significently higher than that of LAK cells (P<0 05).The results indicated that CD 3AK cell is another more efficient killer cell after LAK and TIL cell.更多还原