【摘要】 用斑点杂交（ＤｏｔＢｌｏｔ）方法，检测ＨＢｓＡｇ携带者在接受治疗过程中血清ＨＢＶ－ＤＮＡ存在状况。结果显示，治疗后ＨＢＶ－ＤＮＡ阳性率有明显下降趋势，不同疗程间有明显差异（Ｐ＜０．０１）；随着疗程的延伸，其阳性百分比从高浓度（＞５０ｐｇ）向低浓度（＜５０ｐｇ）移动，直至逐步消失。通过ＨＢＶ－ＤＮＡＤｏｔＢｌｏｔ与ＨＢＶ·Ｍ五项指标（ＥＬＩＳＡ）比较证实，在判断ＨＢＶ是否在体内复制，ＨＢＶ－ＤＮＡＨＢｅＡｇ和Ａｎｔｉ－ＨＢｃ具有高度的一致性。表明ＨＢＶ－ＤＮＡＤｏｔＢｌｏｔ在乙肝治疗效果观察中的应用价值应得到充分的肯定。更多还原

【Abstract】 Dot Blot technique was applied in testing HBV DNA of HBsAg carriers during treatment The result showed that there was a significant decline in the positive rates of HBV DNA after treatment,and there were significant differences among different courses of treatment (P<0 01) Along with the length of the course of treatment,HBV DNA positive percentage shifted from high concentration(>50 pg) to low concentration(<50 pg),until its gradual disappearance The comparision of HBV DNA(Dot Blot) with the five indexes(ELISA) of HBV·M showed that HBV DNA,HBeAg and Anti HBc were highly consistent in judging whether there were any HBV replications in the body The results show that the application of HBV DNA Dot Blot in evaluating the therapeutic effect in hepatitis B treatment possessed pratical value更多还原