【摘要】 报告４６份ＡＦＰ粪便标本用Ｌα、ＲＤ和Ｈｅｐ－２三种细胞作病毒分离，阳性率分别为２６．０９％、６０．８７％、４３．４８％。同步分离出脊灰病毒Ⅰ型３株，Ⅱ型２株，Ⅲ型４株，Ⅰ＋Ⅱ型１株，Ⅰ＋Ⅲ型１株，混合Ｐ２＋Ｅ１株。脊灰病毒总分离阳性率为２８．９５％。非脊灰肠道病毒分离阳性率分别为０％、３６．９６％、２３．９１％。从６份第１次分离结果阴性的高危病例和高度临床病例粪便标本重新分离出１株Ⅲ型脊灰病毒和３株非脊灰肠道病毒。应用Ｌα细胞不仅能作型别鉴定，而且能区别脊灰病毒和非脊灰肠道病毒混合感染更多还原

【Abstract】 Viruses were isolated simultaneously from stool specimens of 46 AFP cases by L α,RD and Hep2 Cells,with positive rate 26 09%,60 87% and 43 48% respectively.A total of 3 strains of poliovirus Ⅰ, 2 strains of poliovirus Ⅱ,4 strains of poliovirus Ⅲ, 1 strain of poliovirus Ⅰ+Ⅱ, 1 strain of poliovirus Ⅰ+Ⅲ and 1 strain of poliovirus Ⅱ+non polio enterovirus were isolated from the specimens.The total isolation rate of poliovirus was 28 95% whereas the isolation rate of non polio enterovirus was 0%,36 96% and 23 91% respectively in the 3 kinds of cells.One strain of poliovirus Ⅲ and 3 strains of non polio enterovirus were re isolated by 22nd generation RD and Hep 2 cells simultaneously from specimens of high risk cases or clinical cases which were negative in the first isolation by 212th generation Hep 2 or RD cells.The application of L α cell can not only identify the type of poliovirus,but also distinguish poliovirus infection from non polio enterovirus infection.更多还原