【摘要】 为探讨人乙肝病毒（ＨＨＢＶ）ＤＮＡ在树鼠句肝细胞内存在状态，对经血清学证明已被感染的树鼠句肝组织进行转移印迹杂交分析。结果，在实验感染早期的树鼠句肝细胞检出ＨＢＶＤＮＡ复制的中间体；在实验中晚期的动物肝细胞发现整合型的ＨＢＶＤＮＡ。表明树鼠句肝对ＨＨＢＶ敏感，可成为乙肝和肝细胞癌发病机理研究及治疗乙肝药物筛选的较好的实验动物模型。更多还原

【Abstract】 The human hepatitis B virus (HHBV) markers in the serum of the experimental tree shrews that had been inoculated with HHBV were detected by radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA).The intermediate form of HHBV replication and integrated HHBV DNA were found in the liver cells of these tree shrews in the early and later experimental stages respectively by southern blot analysis.These results indicated that tree shrews may easily be infected with HHBV and it is a useful animal model to study the drugs for prevention and treatment of hepatitis B and the mechanism of hepatocellular development.更多还原