【摘要】 用高效液相色谱法同时测定尿液中氯氮平和其Ｎ－去甲基活性代谢物去甲氯氮平的浓度，测定方法采用ＯＤＳ柱分离，紫外检测器检测波长为２５４ｎｍ，流动相为甲醇：水：四甲基乙二胺：冰醋酸（６２：３８：０．４５：０．３５，Ｖ／Ｖ），内标为安定。标准曲线范围为０～３０００μｇ／Ｌ，（ｒ＝０．９９９９）。最低检出浓度：氯氮平４μｇ／Ｌ，去甲氯氮平２μｇ／Ｌ，尿液经二次溶剂提取后，氯氮平与去甲氯氮平的绝对回收率大于９０％。日内变异与日间变异氯氮平为０．４～３．８％，去甲氯氮平为１．３～６．３％。本方法灵敏、精确，适用于尿液中氯氮平及去甲代谢物浓度的测定更多还原

【Abstract】 A HPLC method using ODS column and UV detector at 254 nm wavelength has been developed for determining clozapine(CLP) and its N-demethylated metabolite desmethylclozapine (DCLP) in urine. The mobile phase is consisted of methanol:water: tetramethy ethylemediamine:acetic acid(62:38:0.45:0.35 V/V). Diazepam is used as internal standard.Execellent liner relationship was obtained from the range of 0 to 3000 μg/L. The minimal limit of detection was 4 μg/L for CLP and 2 μg/L for DCLP. The recovery rate of CLP and DCLP were better than 90%;Interassay and intraassay evaluation was 0.4 to 3.8% of CLP and 1.3 to 6.3% of DCLP. Practice suggested this method was sensitive,accuracy and suitable for simultaneous determining CLP and DCLP in urine.更多还原