【摘要】 以不同醇或醚为配基，发展了一系列以硅胶为基质的新型疏水色谱固定相，研究了以山梨醇及其单丁醚为配基的填料对生物大分子分离的最佳色谱条件。该固定相可以很高的活性回收率使蛋白质得到较好的分离，同时可以纯化白介素－2、α－干扰素和甲状腺素结合蛋白。更多还原

【Abstract】 A series of hydrophobic interaction chromatographic stationary phases based on silica sphere have been developed. The ligand is alcohol or ether. Chromatographic conditions used for the separation of biopolymer are evaluated. This packing is suitable for the separation of proteins with high recovery of bioactivity and the purification of Transthyretin, IL-2 and α-Interferon.更多还原