【摘要】 对天山根瘤菌群的模式株A－IBS（＝CCBAU3306）16SrDNA的全序列进行了测定，以确定它在系统发育中的地位。实验中设计了6个引物，通过聚合酶链反应扩增天山根瘤菌模式菌株A－IBS的16SrDNA。应用DNA定向测序技术，获得了菌株A－IBS的16SrDNA全序列，将所得序列与根瘤菌已知种的模式菌株16SrDNA全序列一起进行聚类分析，得到了系统发育树状图。在树状图中，菌株A－IBS所代表的根瘤菌群与华癸根瘤菌（R．huakuu）群、鹰嘴豆根瘤菌（R.ciceri）群、地中海根瘤菌（R．mediterrranean）群及百脉根根瘤菌（R．loti）群的亲缘关系较近，与其它根瘤菌群的亲缘关系较远，存在着属水平的差异，表明了这些相近根瘤菌群构成了根瘤菌的一个独立的发育系。更多还原

【Abstract】 Six primers are designed. 16SrDNA of type strain A-IBS (= CCBAU 3306 ) of R. tianshanense is amplified by polymerase chain reaction (PCR) and its sequence from direct DNA sequencing is obtained.Based on the anlalysis of 16SrRNA gene sequence of related sabins, a phylogenetic tree has been produced,which shows that the phylogenetic position of strain A-IBS is closely related to R. huakuii, R. ciceri, R.mediterranean, R. loti and distinct from other species of Rhizobium.更多还原