【摘要】 本文首次报道了一种新的羧酸活性酯试剂，N-羟基琥珀酰亚胺-3-吲哚乙酸酯作为柱前荧光衍生试剂，流动相为10mmol／L柠檬酸-Na2HPO4－pH3．6的甲醇／水（20／80，V／V）溶液，C18柱，于λcx／λcm＝278nm／355nm处进行荧光检测，高效液相色谱分离测定了谷胱甘肽、二甘肽、谷氨酸、胱氨酸、甘氨酸．当S／N＝3时，检出限为0．2至2．5Pmol．更多还原

【Abstract】 A new activated ester fluorescence derivatization reagent, N-hydroxysucci-nimidyl-3-indolylacetate(SIIA) is used for analysis of small peptides and their hydrolysates by HPLC and fluorescence detection. In a one-step procedure, SIIA reacts with peptides and amino acids to form stable derivatives. Studies on derivatization conditions demonstrate ex-cellent derivative properties of the reagent. Maximal yields are observed with a molar reagent excess of approximately twenty five or greater and heated at 60℃ for 10 min in pH 8.0 buffer. SIIA derivatives of glutathionide, diglycine, glutamic acid, cystine and glycine are separated on C18, reverse-column with the mobile phase of CH3OH/H2O (20/80, V/V)containing 10 mmol/L pH 3. 6 citric acid-Na2HPO4 buffer. They are detected at 278 nm/355 nm with the detection limits ranged from 0. 2 pmol to 2. 5 pmol.更多还原