【摘要】 利用反相高效液相色谱／挥发激光散射检测器发展了分析胆汁酸的新方法，采用甲醇和乙酸铵缓冲液混合溶液为冲洗体系，梯度淋洗，１０种甘氨型和牛磺型胆汁酸在２５ｍｉｎ内完成基线分线。方法简单快速，不需衍生，血清样品经预处理，平均回收率为９６．１％，在４～５０ｍｇ／Ｌ浓度范围内对峰面积与浓度进行线性回归分析，回归系数在０．９９１５～０．９９８２之间。可用于正常人和肝硬化病人血清中胆汁酸的测定。更多还原

【Abstract】 A new, simple and rapid method for the analysis of glycine-and taurine-amidated bile acids by reversed-phase high performance liquid chromatography with light scattering detector is developed. A complete resolution of 10 bile acids is obtained within 25 minutes using gradient elution by ammonium acetate buffer-methanol (62.5%75%) binary mobile phase. The calibration curve shows a good linearity in the range of 4~50 mg/L with a corre- lation coefficient higher than 0.99 for individual bile acid. The recovery is 89. 3%-106.9%. This method has been used for the determination of bile acids in human serum of the healthy and hepatocirrhosis patient.更多还原