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测定血红蛋白的一种电化学分析方法
An Electrochemical Method for the Determination of Hemoglobin
【摘要】 血红蛋白可以在微裸银电极上得到电流响应。蛋白质溶液中加入适量的十二烷基硫酸钠,血红蛋白峰电流明显增大,峰形对称,可直接用于血红蛋白的分析测定。实验发现,在5.0×10-7~5.0×10-6mol/L浓度范围,血红蛋白氧化峰电流与其浓度呈正比,用于人体血样中血红蛋白的测定与光度法结果一致。
【Abstract】 Hemoglobin voltammetrically responds at a bare silver microelectrode. By the in- troduction of a proper amount of sodium dodecyl sulfate, the peak current of the protein greatly increased and its voltammogram became symmetrical, so the method could be directly used for the determination of protein. Experimental results revealed that the dependence of peak current on hemoglobin concentration was linear in the range of 5.0 ×10-7- 5. 0 × 10-6 mol/L. The determination for a clinical human blood sample was satisfactory.
【关键词】 电化学分析;
血红蛋白;
微银电极;
十二烷基硫酸钠;
【Key words】 Electrochemical analysis; hemoglobin; silver microelectrode; sodium dodecyl sulfate;
【Key words】 Electrochemical analysis; hemoglobin; silver microelectrode; sodium dodecyl sulfate;
【基金】 国家自然科学基金资助课题。
- 【文献出处】 分析化学 ,Chinese Journal of Analytieal Chemistry , 编辑部邮箱 ,1997年01期
- 【分类号】O657.1
- 【被引频次】26
- 【下载频次】421