【摘要】 建立了高效液相色谱法（HPLC）测定云南红豆杉原始提取液中紫杉醇含量的方法,经比较C-（18）和氰基固定相,发现在氰基柱上,以MeOH—MeCN-H₂O（20:16:64体积分数）为流动相时,紫杉醇获得更满意的分离,流速2.0mL/min,柱温40℃,UV检测波长:227nm。方法简便、准确、适合于微量紫杉醇的测定。更多还原

【Abstract】 A reverse phase high performance liquid chromatographic method for the determination of taxol in ethanol extract of the bark of Taxus yunnanensis Cheng et L. K. Fuis described. In comparison with the Shim-pack CLC-ODS column, a 5μm Shim-pack CLC-CN column(6.0 mm i. d.x150 mm) was more suitable for the separation of taxol from the extract. The eluant was consisted of methanol, acetonitrile and water in 20:16:64 volume ratio. The flow rate was 2.0 mL/min and the column oven tempamture was controlled at 40℃. The effluent was monitored at 227 nm. The described method is simple and accuate and has the potential for direct quantitation of minor taxol in crude ethanolic taxol extracts.更多还原