【摘要】 目的探讨３Ｈ－ＴｄＲ参入ＤＮＡ技术在组织培养中的合理应用。方法测定１６周龄ＳＨＲ和ＷＫＹ大鼠培养的胸主动脉血管平滑肌细胞（ＡＭＳＣ）的自然增长曲线以及接种后６０ｈ期间，每隔４～６ｈ３Ｈ－ＴｄＲ参入的变化。结果ＳＨＲ大鼠ＡＳＭＣ分裂增殖能力比ＷＫＹ强，在１５％ＦＢＳ－１６４０培养基作用下，１～６０ｈ期间，ＳＨＲＡＳＭＣ３Ｈ－ＴｄＲ高峰参入率在２０～２４、５０～５５ｈ之间，ＷＫＹ的高峰参入率在４５～５０ｈ之间。结论要使３Ｈ－ＴｄＲ参入能真实反映培养细胞的ＤＮＡ合成和增殖水平，需根据特定细胞株在某种特定药物干预下的细胞周期，选择适当的３Ｈ－ＴｄＲ标记期，使实验设计更合理。更多还原

【Abstract】 Aim\ To explore the rational apPlication of 3H TdR incorporation in tissue culture.\ Methods\ Aortic smooth muscle cells(ASMC) from 16 week old SHR and WKY were cultured to test the growth curves and the changes of 3H TdR incorporation every 4～6 hours in a period of 60 hours.\ Results\ SHR ASMC had stronger proliferative ability compared with WKY. The peak incorporative rate of 3H TdR of SHR ASMC occurred between 20～24,50～55 h while WKY between 45～50 h.\ Conclusion\ Proper 3H TdR labelling period should be chosen according to the cell cycle of specific cell line intervened by certain medicines so that the level of DNA synthesis and proliferation can be authentically reflected by 3H TdR incorporation and the experiment design more rational.更多还原