【摘要】 为探讨内皮依赖性舒张因子（EDRF）对耳蜗微循环的调节作用，本实验应用活体显微镜结合电视摄象的图像分析技术及血管纹螺旋韧带铺片技术，观察了不同剂量EDRF合成前体L-精氨酸（L－Arg）及EDRF的合成抑制剂L-硝基-精氨酸（L－NNA）对耳蜗血流（cochlearbloodfrow，CoBF）的作用。结果显示：L－Arg100mg／kg组动物在给药5分钟后耳蜗血管流速开始加快，20分钟时平均增加达31．4％，血管扩张，平均30分钟恢复正常；L－Arg200mg／kg组动物，在给药早期耳蜗血管纹血流速度增加，之后流速减慢，20分钟时平均下降54％，并伴BP下降；而L－NNA组给药后血流速度减慢，血管收缩；同时对EDRF增加CoBF的机理进行了探讨。提示：①、EDRF对CoBF有调节作用；②适当剂量的EDRF可扩张血管，加快血流，增加耳蜗微循环的灌注量。更多还原

【Abstract】 Intravital microscopic examination with image processing technique and the prepa-ration of stria vascularis were undertaken to investigate the role of endothelium-derivedrelaxing factor (EDRF) in cochlear blood flow (CoBF). Animals were administrated bydifferent dose L-arginine which was the substance of EDRF’s synthase; and a competi-tive inhibitor of EDRF synthase by NG-nitro-L-arginine (L-NNA). The results showedthat EDRF induced a sequence of changes in CoBF. In animals of administrated(100mg/kg) group, CoBF resulted in increased in 5 min; and followed by a gradual in-crease above baseline (mena= 31. 4%) in 20 min. This CoBF increase and subsequentrecovery were associated with significant change in vessel diameter of stria vascularis. Inanimals of administrated (200mg/kg) group, CoBF resulted in increase in early period ;then followed decreased in 10 min (mean=54%) and decreased in BP as well, With in-travenous administrated L-NNA (10mg/kg), both velocity and diameter were reducedby intravenous application of L-NNA (30mg/kg). Our study demonstrated that: (1)CoBF might be regulated by EDRF in guinea pig; (2) EDRF might be used to cure innerear disorder by dilating vessels and incresing velocity.[Chinese Arch Otolaryngol Head Neck Surg, 1997; 4 (3): 172～176更多还原