【摘要】 本研究合成了伪狂犬病毒糖蛋白ｇｐ５０基因引物，该引物能够扩增糖蛋白ｇｐ５０基因中４３４～６５１之间的２１７ｂｐＤＮＡ片段，该片段含ＳａｌⅠ酶切位点，应用引物对几种不同的伪狂犬病毒株多聚酶链式反应（ＰＣＲ）扩增结果全为阳性。对自然发病猪、潜伏感染猪、伪狂犬病毒引起的死胎等４６头猪１６１个样品进行了ＰＣＲ扩增，其中４６头猪７１个样品ＰＣＲ扩增结果为阳性。羊口疮病毒（ＯＲＦＶ）、牛传染性鼻气管炎病毒（ＩＢＲＶ）、猪细小病毒（ＰＰＶ）及正常细胞对照ＰＣＲ扩增的结果均为阴性更多还原

【Abstract】 A pair of primers within pseudorabies virus glycoprotein gp50 gene was synthesized to amplify specifically a short DNA fregment (217bp) with one Sal I site. When the gnome DNA of various PRV Strains were used to amplify, all the results were positive,The 161 samples from 46 pigs, including naturally infected pigs,experimently infected pigs and stillborn fetus resulting from PRV,were used to amplify,the results of 71 samples from 46 pigs were positive,but the control samples of ORFV,IBRV,PPV ,BHK 21 Cell Were Negative.更多还原