【摘要】 从杂色曲霉(Aspergillus versicolor)和构巢曲霉(Aspergillus nidulans)培养物中提取分离到一种淡黄色针状结晶,经有机波谱分析,证实为杂色曲霉素(sterigmatocystin,ST)。ST粗提物经紫外薄层扫描进行ST定量测定,给试验骡每天投服ST 4mg/kg(第1～10天)、8mg/kg(第11～36天)。试验骡于第17天开始出现中毒症状;于第37天,4匹试验骡中有3匹死亡。其中毒症状与死后剖检变化与宁夏盐池、灵武两县马属动物"黄肝病"基本一致。血液常规检查表明:分叶核中性白细胞上升,淋巴细胞下降。血液生化检查发现:血清GOT、GPT、AKP、LDH活性升高。BUV含量上升,血清总蛋白、白蛋白及球蛋白含量下降。剖检结果表明皮下及内脏黄染,并伴有广泛性出血。病理组织学变化以肝细胞,肾小管上皮细胞和大脑神经细胞空泡化为特征。从而认为宁夏多年来所发生的马属动物"黄肝病"主要是ST中毒引起。更多还原

【Abstract】 Apale yellow needle—like crystal was extracted from the cultured materials of Aspergillus versicolor and A. nidulans and identified by organic spectrums to be sterigmatocystin(ST). The ST content in the crude extracts was measured by ultraviolet TLC scanner. 4mg/kg of ST was orally administrated to each of the four mules daily for the first 10 days, 8mg/kg for following 26 days. on day 17 of the administration, the animals showed toxic symptoms;on day 36 of the exepriments, 3 of the 4 animals were died. The toxic symptoms and necropsy changes were very similar to the "yellow liver disease" in two counties of Yan Chi and Lin Wu of Ning Xia. The regular blood examinations showed that the number of neutrophlis was incraesed and lymphocytes decraesed. The blood biochemcal examinations indicated that the activities of serum GOT, GPT, SDH, AKP, LDH were elevated and the concentration of BUN ineraesed. The concentration of total protein, albumen and globin in serum were declined. The necropsy showed a yellow colour in subcutaneous tissues and internal organs accompanying with intensive hemorrhage. Histopathological findings characterised by the vaculation of liver, renal tubular epithelia and cerebrum cells. Thus,it is believed that "yellow liver dissase", having been reported for many years in Ning Xia, is mainly caused by ST poisoning.更多还原