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水分胁迫对玉米类囊体膜组分的影响

EFFECTS OF WATER STRESS ON THYLAKOID MEMBRANE COMPONENTS IN ZEA MAYS L

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【作者】 郝利民; 王洪亮; 粱厚果;

【Author】 Hao Limin; and Wang Hongliang (Department of Biology, Lanzhou University, Lanzhou 730000)Liang Houguo (Department of Biology,Sichuan University, Chengdu 610064)

【机构】 兰州大学生物系!兰州; 730000; 四川大学生物系!成都; 610064;

【摘要】 研究了水分胁迫对玉米(ZeamaysL,新玉4号)叶片类囊体膜的蛋白、色素组分,以及PSⅠ、PSⅡ和Cytb/f复合物的影响。类囊体膜蛋白电泳显示水分胁迫使总膜蛋白含量下降。高效液相色谱分析表明水分胁迫也引起类囊体膜色素成分的改变。通过测定P700及各种细胞色素的含量计算所得的PSⅠ、PSⅡ和Cytb/f复合物成分表明PSⅠ对水分胁迫不敏感,Cytb/f复合物在严重水分胁迫时才受到影响,而PSⅡ在中度和严重水分胁迫时均受到较明显的影响。

【Abstract】 The effects of water stress on the components of protein,pigment,and photosystem Ⅰ (PS Ⅰ ),Photosystem Ⅱ (PS Ⅱ ) and Cyt b/f complexes of thylakoid in Zea mays L.(Xinyu, No. 4) were studied in present paper. The electrophoretic photograph showed that the thylakoid membrane proteins were reduced by water stress. Analysis by high pressure liquid chromatography (HPLC) showed that water stress causes the changing of pigment components in thylakoid. The components of PS Ⅰ,PS Ⅱ and Cyt b/f complexes in thylakoid, which calculated by determining the contents of P700 and cytochromes,showed that PS Ⅰ is not sensitive to water stress,Cyt b/f complex is affectedonly by severe water stress,however,PS Ⅱ is affected both by moderate and severe water stress.

【关键词】 玉米; 水分胁迫; 类囊体膜;
【Key words】 Zea mays L.; water stress; thylakoid membrane;
【基金】 国家自然科学基金;博士点基金
  • 【文献出处】 西北植物学报 ,ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA , 编辑部邮箱 ,1997年06期
  • 【分类号】Q945
  • 【被引频次】2
  • 【下载频次】110
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