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反向被动血凝试验判定黄鼠、沙土鼠鼠疫阳性诊断标准的商讨
Discussion on the Positive Diagnosing Standard for Citellus and Meriones Plague with the RPHA
【摘要】 在陕西、宁夏、内蒙收集长爪沙土鼠、达乌尔黄鼠和阿拉善黄鼠健康脏器(肝、脾)标本685份,经鼠疫反向被动血凝试验(RPHA)检查,出现72分非特异反应,反应值均在1:10和1:20以下。33份阳性参照标本(腺肿穿刺液)滴度在1:20~1:5120之间。经统计学百分位数法求得RPHA正常范围上限值约为1:10(P=O.999),从而把≥1:20稀释度定为RPHA的阳性诊断标准。相比之下,《鼠疫防治手册》把≥1:100稀释度定为RPHA阳性诊断标准是过高了。
【Abstract】 Of 685 organ samples collected from the healthy citellus dauricus,Citellus alashanicus and meriones unguiculatus in Shanxi,Ningxia,and Inner mangolia provinces tested with the method of plague reverse passive hemagglutination (RPHA), 307 samples showed non-specific reactions with the titre of less than 1: 1O,and 33 samples of the adenoncus puncture fluid had positive results with the titres between 1: 20and 1: 5120,The threshold value was 1: 10,which was calculated through the percentile method. Therefore,the titre for positive diagnosing standard would be set up as ≥ 1: 2O rather than ≥1: 100 drafted in 《Plague Manual》.
- 【文献出处】 地方病通报 ,ENDEMIC DISEASES BULLETIN (CHINA) , 编辑部邮箱 ,1997年01期
- 【分类号】R516.8
- 【被引频次】2
- 【下载频次】16