【摘要】 为人白细胞介素-10（hIL-10）的理论研究和临床应用研究提供材料。方法：利用基因工程技术，将hIL-10cDNA克隆在大肠杆菌表达载体中，在大肠杆菌中高效表达含凝血酶识别序列的hIL-10的融合蛋白。表达蛋白经凝酶消化后，可去除MS2细菌蛋白。结果：获得高纯度的非融合型rhIL-10。结论：活性分析表明rhIL-10具有抑制LPS刺激的外周血单个核细胞产生IL-6的能力。更多还原

【Abstract】 Objective: In order to provide necessary material for future theory and clinic study of IL-10.Methods: The cDNA of human interleukin-10 (hIL-10) was cloned in E. coli expression vector by genetic engineering technique. MS2-IL-10 fusion protein could be overexpressed in E. coli. The MS2 bacteria protein could be removed by thrombin digestion. Results: Purified hIL-10 was obtained. Conclusion: Bioactivity assay revealed that rhIL-10 could inhibit the exprereion of IL-6 by PBMC induced with LPS.更多还原