【摘要】 目的：比较不同诱导方法对LAN细胞杀伤活性的影响。方法：外周血单个核细胞（PBMC）用重组白细胞介素-2（rIL-2）体外诱导LAK细胞，采用3H－TdR释放法测定LAK活性。结果；（1）PBMC诱导3h后，LAK活性高于或至少不低于24～72h者；（2）PBMC诱导3h后洗去游离的rIL-2，并不影响继续诱导LAN细胞活性；（3）PBMC分离后以清洗2次为佳；（4）PBMC用正常人血浆培养比用肝炎病人自体血浆培养诱导的LAK活性高。结论：在本试验系统中，PBMC分离后，清洗2次，以正常人血浆经IL-2诱导3h为最佳回输条件。更多还原

【Abstract】 Objective: To compare LAK activities induced by different methods in vitro. Methods: PBMC waswashed with varying times and induced by rIL-2 for different length of time. LAK activities were measured by3H-TdR release assay. Results: The LAK activity induced by rIL-2 for 3 hours was higher than that fortraditional 24 ~ 72 hours. When the free-IL-2 in the cells was removed after the inducing, the LAK activitystill remained at least for three days. The highest activity was reached when PBMC was washed twice. TheLAK activity of PBMC cultured in normal plasma was higher than that in the plasma of patient with hepatitis.Conclusion: This study provides a simple, time-saving method to induce LAK activity in vitro.更多还原