【摘要】 目的：建立一种可同时分泌多巴胺（DA）和GDNF的工程细胞，并研究其在帕金森病（PD）基因治疗中的可能作用。方法：克隆携带Kozak序列的GDNFcDNA，转染至可分泌DA的MN9D细胞系，将此工程细胞和大鼠原代多巴胶能神经元共培养，免疫组化检测DA能神经元。结果：发现该工程细胞可防止DA能神经元退变死亡，并有助于DA能神经元抵抗MPP+的毒性损伤。结论：本文首次将PD防治兼顾策略结合起来构建MN9D工程细胞，结果表明其在PD的基因治疗中可能具有重要的应用价值。更多还原

【Abstract】 Objective: To set up a kind of engineered cells that can secrete doparnine (DA) and GDNF at thesame time, and study its possible effectS on gene therapy of Parkinson’s disease. Methods: Clone the GDNFcNDA with Kozak sequence by RT-PCR, and then trans fect it into MN9D cell line that is a hybriddopaminet-gic neuronal cell line and can secrete DA, co-culture the engineered cells with primary mesercephaliccells Results: The engineered cells showed obvious protective effects such as anti-degeneration and anti-MPP+ lesion. GDNF produced by the engineered MN9D cells is of full biological fuction. Conclusion: Thepresent study first set up the engineered MN9D cells based on the strateqy of combining prevention withtherapy, The results show that they could play an important role in gene therapy of PD.更多还原