【摘要】 目的：研究人衰老成纤维细胞c-fos基因染色质结构状态。方法：DNaseⅠ敏感性分析法。结果：衰老细胞c-fos基因在表皮生长因子（EGF）诱导后对DNaseⅠ的敏感性无明显变化，而年轻细胞对DNaseⅠ的敏感性显著升高。结论：衰老细胞的c-fos基因可能在异染色质上，处于关闭状态，因而不易为EGF所诱导。更多还原

【Abstract】 Objective:To analyse the state of chromatin structure and c-fos gene local chromatin insenescent fibroblasts. Methods: DNase Ⅰ sensitivity assay, Southern blot.Results: The chromatin inyoung cells were more sensitive to DNase Ⅰ than that in aging cells, the chrornatin in EGF-treated cellswere more sensitive to DNase Ⅰ than that in control groups. The c-fos gene local chromatin structure inaging cell after EGF-treatment was resistent to DNase Ⅰ as compared to that in young cells.Conclusion: The c-fos gene of human senescent fibroblasts maybe located in heterochromatin.更多还原